The Problem of Pain
Chronic pain is pervasive and afflicts 1 in 5 adult Canadians. As the population ages and modern medicine allows people to survive serious illness, that number is expected to swell to 1 in 3. Chronic pain also affects young children, with 6–8% of children in Canada living with intense and frequent pain. That the problem of chronic pain persists, and is growing, is striking and highlights a major gap in our understanding of the fundamental neurobiology of pain.
Our work aims to answer key questions such as: How does our body tell us something is painful? Why do certain people go on to develop chronic pain whereas others, with apparently similar disorders or injuries, do not? Why is pain so difficult to treat? And how do opioids, which are the mainstay of pain therapy, cause their side effects?
To begin to unravel the paradox of pain, our research focuses on discovering the fundamental molecules and processes involved in chronic pain and enhancing the utility of opioid drugs in treating pain conditions. A strong focus of our research is the role of an understudied class of cells known as microglia, which are immune cells in the central nervous system, and the complex interplay between microglia and neurons in chronic pain and opioid analgesia.
Our Research Themes
1) Neurobiology of acute and chronic pain
2) Neurobiology of microglia (immune cells in the brain and spinal cord)
3) Opioid therapy and unwanted side effects associated with opioids
4) Arthritis Pain
To study the cellular mysteries of pain, we use a range of techniques that encompass biochemical, molecular, live cell imaging, and behavioural manipulations. Cutting edge technology enables us to employ an integrative and comprehensive approach to dissect the key genes and proteins that drive chronic pain at the single cell and whole systems level.
Dr. Tuan Trang
Dr. Trang obtained his BSc (Honours) and PhD degrees from Queen’s University. His doctoral training in Pharmacology and Toxicology focused on the effects of opioid drugs in the brain and spinal cord. He was then a CIHR Postdoctoral Fellow at the Hospital for Sick Children in Toronto, where he expanded his area of expertise into the study of chronic pain. Dr. Trang is currently an Associate Professor in the Department of Comparative Biology and Experimental Medicine (Faculty of Veterinary Medicine) and the Department of Physiology and Pharmacology (Cumming School of Medicine). He is also a full member of the Hotchkiss Brain Institute, and an associate member of the Alberta Children's Hospital Research Institute. Dr. Trang is the recipient of a CIHR New Investigator Award and he is a Rita Allen Foundation Scholar.
Dr. Trang enjoys playing Star Wars with his son, reading books with his daughter, and playing tug of rope with his crazy Labrador Retriever. Pre-kids he was actively involved in basketball, tennis, and martial arts.
Contact: (403) 220 8357 | email@example.com
Nicole Burma, PhD
Leaders in Medicine MD/PhD Student
Nicole acquired her BSc (Biology, Co-op) from the University of Victoria and started her graduate training in the Trang Lab in July 2013. Nicole defended her PhD in April 2018 and has joined the MD class of 2021. Nicole's research focuses on the role of microglia in opioid withdrawal using behavioural, biochemical, and live-cell imaging approaches. Outside of the lab, Nicole enjoys skiing, pottery, home decorating and cuddling with her kitty Mumu. She also loves to travel, particularly to tropical destinations.
Churmy Fan, MSC
Originally from Vancouver, Churmy completed her MSc in Cell and Developmental Biology at the University of British Columbia. She is currently pursuing her PhD in Neurosciences in the Trang Lab, investigating microglial mechanisms in chronic pain. Outside the lab, Churmy is actively involved in the community as a co-founder of the Boss Dodgeball League. In her spare time, Churmy enjoys taking care of her reef aquarium, and doing agility with her dogs Bui Bui and Penny.
Charlie Kwok, PHD
Charlie completed her PhD studies with Dr. Gareth Hathway and Prof. Victoria Chapman at the University of Nottingham, U.K., and her first post-doctoral training with Prof. Maria Fitzgerald at University College London. She joined the lab in November, 2016. She will be researching the short and long term consequences of pain and opioid treatments during early life, and the relationship between chronic pain and motor functions. Outside of the lab she likes to run, cook and travel. She has toured all over Asia and Europe and is looking forward to new adventures in North America.
Kristina Komarek, HBSc
Kristina completed her HBSc with a Neuroscience Specialist and a Biology Minor at the University of Toronto. She has returned to the Trang Lab once again as a summer student and is currently investigating the role of microglial pannexin-1 channels in opioid withdrawal. Outside the lab, Kristina enjoys playing many different board games, skiing at resorts across the Rockies, baking sweet treats and exploring the great outdoors!
Heather Leduc-Pessah, PHD
Leaders in Medicine MD/PhD Student
Originally from Ottawa, Heather completed a BSc in Biochemistry at Queen's University and is excited to be continuing her studies at the Hotchkiss Brain Institute! Heather defended her PhD in May 2017, and is now working towards her MD at the University of Calgary. Heather's research investigates the role of microglia in response to morphine, as well as the role of microglial P2X7Rs in the development of analgesic tolerance. Outside the lab, Heather enjoys an active role in the Leaders in Medicine program, hiking and skiing, and volunteering at the Children’s Cottage Society.
Mike Mousseau, PHD
Mike received his BSc in Neuroscience from St. Lawrence University, New York. Following graduation, he pursued a post-bachelor research opportunity in Washington, DC, working for the US Department of Defense studying traumatic brain injury. Mike completed his PhD in the Trang lab, where he investigated the role of the central nervous system in chronic arthritis pain. Away from the lab, he enjoys watching his hometown Ottawa Senators and keeping active playing recreational hockey. At home, Mike enjoys spending time and going to the park with his dog Mika.
MiCHAEL ZEEMAN, HBSc
Michael completed his BHSc in Biomedical Sciences from the University of Calgary and began his MSc training in the Trang Lab in September of 2018. His research project investigates the effects of drug tolerance on respiratory depression resulting from opioid use. In his spare time, Michael is active with fitness and martial arts, as well as advocating for mental health awareness.
Born and raised in Kelowna, BC, Jeff is currently pursuing a BSc in Neuroscience at the University of Calgary. He joined the lab in the summer after completing his third year, studying the role of exercise on chronic pain and motor recovery following nerve injury. His research interests include cellular and behavioural neuroscience. After his degree, he hopes to enter graduate school. Outside of the lab, Jeff enjoys playing/following hockey, skiing, playing guitar, watching movies, and spending time with friends.
Hailing from Victoria BC, Sydney is pursuing her BSc in Cell Biology at the University of Calgary. Sydney is currently a summer research assistant but will be continuing on in her undergraduate thesis investigating the central mechanisms of post-operative pain. She has spent her previous summers involved with research on the neuroArm team and internationally at the University of Heidelberg. While not in classes or in the lab, you can find Sydney on the softball diamond playing, coaching, or watching. Sydney enjoys baking, rollerblading and volunteering with Special Olympics Calgary.
Where are they now?
Rebecca Dalgarno (defended 2017)
Dr. Nikita Burke
Cynthia Xu, MSc Student (defended 2017)
Rachel Redick, Summer Student (2017)
Ally Pilapil, MSc Student (defended 2016)
Zoe Cairncross, Summer Student (2014, 2015)
Jordan Haidey, Summer Student (2015)
David Lau, Summer Student (2014)
Joanna Rybicka, Summer Student (2013)
Occupational Therapy, University of Alberta
Clinical Manager, National University of Ireland
Medical School, University of Manitoba
Veterinary School, University of Calgary
Ember Dance Company
MSc of Public Health, University of Toronto
MSc in Neuroscience, University of Calgary
BSc in Neuroscience, University of Calgary
Mousseau, M., Burma, N.E., Lee, K., Leduc-Pessah H., Kwok, C.H.T., Reid, A.R., O'Brien, M., Sagalajev, B., Stratton, J.A., Patrick, N., Stemkowski, P.L., Biernaskie, J., Zamponi, G.W., Salo, P., McDougall, J.J., Prescott, S., Matyas, J.R., Trang, T. (2018). Microglial pannexin-1 channel activation is a spinal determinant of joint pain, Science Advances, eaas9846.
Dalgarno, R., Leduc-Pessah H. (co-first author), Pilapil, A., Kwok, C.H.T., Trang, T. (2018). Intrathecal delivery of a palmitoylated peptide targeting Y382-384 within the P2X7 receptor alleviates neuropathic pain, Molecular Pain, 14: 1744806918795793.
Mapplebeck, J.C.S., Dalgarno, R. (co-first author), Moriarty, O., Beggs, S., Tu, Y., Kwok, C.H.T., Mogil, J.S., Trang, T., Salter, M.W. (2018). Microglial P2X4R-evoked pain hypersensitivity is sexually dimorphic in rats, Pain, 159(9):1752-1763.
Stratton, J.A., Holmes, A., Rosin, N., Sinha, S., Vohra, M., Burma, N.E., Trang, T., Midha, R., Biernaskie, J., (2018). Pro-regenerative macrophages regulate Schwann cell dynamics following nerve injury, Cell Reports, 24(10):2561-2572.
Leduc-Pessah H., Weilinger, N.L., Fan, C.Y., Burma, N.E., Thompson, R.J., Trang, T. (2017). Site-specific regulation of P2X7 receptor function in microglia gates morphine analgesic tolerance. Journal of Neuroscience, 37(42):10154-10172.
Burke, N.N., Trang, T. (2017). Neonatal injury results in sex-dependent nociceptive hypersensitivity and social behavioural deficits during adolescence, without altering morphine response, Journal of Pain, 18(11):1384-1396.
Burma, N.E., Leduc-Pessah, H., Trang, T. (2017). Genetic deletion of microglial pannexin-1 attenuates morphine withdrawal, but not analgesic tolerance or hyperalgesia in mice, Channels, 11(5):487-494.
Burma, N.E., Bonin, R.P., Leduc-Pessah, H., Baimel, C., Cairncross Z.F., Mousseau, M., Vijaya Shankara, J., Stemkowski, P.L., Baimoukhametova, D., Bains, J.S., Antle, M.C., Zamponi, G.W., Cahill, C.M., Borgland, S.L., De Koninck, Y., Trang, T. (2017). Blocking microglial pannexin-1 channels alleviates morphine withdrawal in rodents, Nature Medicine, 23(3):355-360.
Leduc-Pessah, H., Burke, N.N., Cahill, C.M., Trang, T. (2016). Opioid-mediated neuroimmune modulation: the central battlefront. Primer of PsychoNeuroImmunology Research. (Editor: Marc Opp) Chapter 41, p317-324.
Mandadi, S., Leduc-Pessah, H., Hong, P., Ejdrygiewicz, J., Sharples, S.A., Trang, T., Whelan, P.J. (2015). Modulatory and plastic effects of kinins on spinal cord networks, The Journal of Physiology, 594(4):1017-36.
Trang, T., Al-Hasani, R., Salvemini, D., Salter, M.W., Gutstein, H., Cahill, C.M. (2015). Pain and poppies: the good, the bad, and the ugly of opioid analgesics, Journal of Neuroscience, 35(41):13879-88.
2014 & earlier
Mattioli, T.A., Leduc-Pessah, H., Skelhorne-Gross, G., Nicol, C.J.B., Milne, B., Trang, T., Cahill, C.M. (2014). Toll-like receptor 4 mutant and null mice retain morphine induced tolerance, hyperalgesia and physical dependence, PLOS One, 9(5): e97361.
Ferrini, F., Trang, T. *(co-first author), Mattioli, T., Laffray, S., Del’Guidice, T., Lorenzo, L., Castonguay, A., Doyon, N., Zhang, W., Godin, A., Mohr, D., Beggs, S., Vandal, K., Beaulieu, J., Cahill, C., Salter, M.W., De Koninck, Y. (2013). Morphine hyperalgesia gated through microglia-mediated disruption of neuronal chloride homeostasis, Nature Neuroscience, 16(2):183-192. [Faculty of 1000 Prime, Must Read]
Sorge, R., Trang, T. *(co-first author), Dorfman, R., Smith, S., Beggs, S., Ritchie, J., Austin, J., Zaykin, D.V., Vander Meulen, H., Costigan, M., Herbert, T.A., Yarkoni-Abitbul, M., Tichauer, D., Livneh, J., Gersh, D., Zheng, M., Tan, K., Johns, S.L., Slade, G.D., Jordan, J., Woolf, C.J., Peltz, G., Maixner, W., Diatchenko, L., Seltzer, Z., Salter, M.W., Mogil, J.S. (2012). Genetically determined P2X7 receptor pore formation regulates variability in chronic pain sensitivity, Nature Medicine, 18(4):595-9.
Beggs, S., Trang, T., Salter, M.W. Microglia and pain. Neuroglia, Third Edition. (ed. Helmet Kettenmann and Bruce Ransom) Oxford University Press, Chapter 68, 2012.
Trang, T., Beggs, S., Wan, X., Salter, M. (2009). P2X4R-receptor mediated synthesis and release of brain-derived neurotrophic factor in microglia is dependent on calcium and p38-mitogen-activated protein kinase activation, Journal of Neuroscience, 29(11): 3518-3528.
McNaull, B., Trang, T., Sutak, M., Jhamandas, K.H. (2007). Inhibition of tolerance to spinal morphine analgesia by low doses of opioid receptor antagonists, European Journal of Pharmacology, 560(2-3): 132-41.
Beggs, S., Trang, T., Salter, M.W. Role of ATP and microglia in enhanced pain states. Immune and Glia Regulation of Pain. (eds. Linda Watkins, Joyce DeLeo, and Linda Sorkin) International Association for the Study of Pain, pp. 283-295, 2007.
Trang, T., Chabot, J.-G., Ma, W., Quirion, R., Jhamandas, K.H. (2006). Spinal modulation of calcitonin gene-related peptide by endocannabinoids in development of opioid physical dependence, Pain, 126: 256-271.
Trang, T., Quirion, R., Milne, B., Jhamandas, K. Role of pain transmitters and arachidonic acid-derived metabolites in the development of opioid tolerance and physical dependence. Recent Developments in Pain Research. (ed. Anna Capasso) Research Signpost, pp. 137-153, 2005.
Trang, T., Quirion, R., Jhamandas, K.H. (2005). The spinal basis of opioid tolerance and physical dependence: Involvement of calcitonin gene-related peptide, substance P, and arachidonic acid-derived metabolites, Peptides, 26(8): 1346-1355.
Trang, T., McNaull, B., Sutak, M., Quirion, R., Jhamandas, K.H. (2004). Involvement of spinal lipoxygenase metabolites in hyperalgesia and opioid tolerance, European Journal of Pharmacology, 491: 21-30.
Trang, T., Sutak, M., Quirion, R., Jhamandas, K.H. (2003). Spinal administration of lipoxygenase inhibitors suppresses behavioural and neurochemical manifestations of naloxone-precipitated opioid withdrawal, British Journal of Pharmacology, 140(2): 295-304.
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The Trang Laboratory
The University of Calgary
Hotchkiss Brain Institute
3330 Hospital Dr NW
Calgary, AB, T2N 4N1
Please contact Dr. Trang for more information about our research and available lab positions:
(403) 220 8357 | firstname.lastname@example.org